| Real Time PCR Machine |
| Features:Flexible five-color detection systems for easily calibrating dyes without requiring the addition of new filter sets or better.Advanced optical multicomponenting algorithm minimizes spectral cross with superior for multiplexing.Customizable 21 CFR Part 11 module.Export to JPEG and Power Point, configurable graphs & plots.Advance Real-Time PCR System uses fluorescence-based polymerase chain reaction (PCR) reagents to provide.Quantitative detection of target nucleic acid sequences (targets) using real-time analysis. |
| Qualitative detection of targets using post-PCR (endpoint) analysis.Qualitative analysis of the PCR product (achieved by melt-curve analysis that occurs post-PCR).Should be capable to use to run assays designed with custom dyes. Custom dyes must fluoresce within the 520 to 650 nm (Approx.) spectral range measured instrument.Real-Time PCR System capable of powering off and stored for extended period. The length of the period of inactivity determines the method used to power off the instrument. |
| Technical Specifications: |
| Brand | : Applied Biosystems by Thermo Fisher Scientific. |
| Model | : Quant Studio 5. |
| Sample capacity: | Individual tube, 8 strips and 96 well plates (0.2 mL). |
| Sample size: | 20–100μl (Approx.). |
| Excitation range: | 455–700 nm (Approx.). |
| Optics: | 5 emission filters, 5 excitation filters, CCD Camera. |
| Light source: | Tungsten Halogen Lamp. |
| Optical detection: | CCD camera. |
| Detection range: | 455–700 nm (system can detect dyes such as SYBR, FAM, JOE, VIC, Cy3, TAMRA, NED, ROX, Texas Red, Cy5). |
| Multiplex capability: | Up to 5 targets. |
| Dynamic Range: | min. 9 logs of linear dynamic range. |
| Peak Block Heating Rate: | 2.5°C/sec (Ave ramp rate). |
| Heating & cooling method: | Peltier. |
| Temperature Range: | 4°C –100°C. |
| Temperature Accuracy: | ±0.25°C of set point/display temperature. |
| Temperature Uniformity: | ±0.5°C, 30 sec after clock start. |
| Calibrated Dyes at Installation: | SYBR® Green I, ROX™, TAMRA™, Texas, Red®, VIC™, JOE™, FAM™, Cy5™, Cy3™, NED™ |
| Passive Reference Dyes: | ROX dye or any calibrated dye. Use of a Passive reference dye is optional. |
| Sensitivity: | Detection of one copy of template in a 50uL reaction for a single reporter TaqMan® assay, with 99.7% Confidence. |
| Multiplex analysis: | Up to 5 targets per well. |
| Sample ramp rate: | ±1.1⁰C/ sec (Approx.). |
| Thermal cooling system: | Peltier based system. |
| Throughput: | 4 to 5 96-well plates per 8-hr day. |
| Program control: | Software/computer based. |
| Real time PCR Detection System/Software must be included: |
| Peltier based 96-well thermal cycling block. |
| Five Plex Multiplexing System. |
| Halogen lamp and CCD camera. |
| Installation and basic operator instruction by certified Field Service Engineer (Certificate to be provided). |
| Software Specification: |
| Primer / probe design software. Custom design primers and probes.Software supported Applications: Comparative Ct, Gene expression analysis, Absolute Quantitation, Relative Quantification, SNP Genotyping, Allelic Discrimination, End point detection, Isothermal and Plus/Minus assays.At least four safety alert words appear in user documentation at points in the document where need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies in a particular level of observation or action. |
| Accessories: | a) Optical 8-Tube Strip, 125 strips/pack; Qty – 01 |
| b) Optical 8-Cap Strips, 300 strips/pack; Qty – 01 |
| c) Optical Adhesive Film, 100 covers/pack; Qty – 01 |
| d) Optical 96-Well Reaction Plate, 10 plates/pack; Qty – 01 |
| e) Master mix with dye- 1 set |
| f) Brand Laptop Computer, Intel Core i5, 8GB RAM, 500 GB SSD Hard disk, DVD-RW ± Dual, Keyboard, Mouse & Pre-installed Microsoft Original Windows-10 Professional, 22” full screen monitor- 1No. |
| g) Laser Jet Printer with auto duplex system- 1No. |
| h) An installation Table with cabinet and rack included. |
| Other Accessories: | Required necessary accessories & consumables quoted avobe and will be supplied with equipment to install & operate the system. |
| Country of Origin | USA. |
| Make and assembly | Singapore. |
| Certification- Country of Origin | Yes |
| Manufacturer Authorization Letter | : Yes (On Manufacturer’s letterhead pad). |
| Manufacturer Warranty and guaranty | : Yes (On Manufacturer’s letterhead pad). |
| Standard Warranty and guaranty | : One Year. |
| Extended Service Warranty | : Five years (including warranty period). |
| Training | 2-3 days training on maintenance, trouble shooting, operation, and application of equipment will be provided by us after installation and commissioning and will provide 2-days training after 3-months of 1st schedule training at user site. We will train up the appointed scientists/experts/technician until ready for operation. |
| Packing list | Packing list from manufacturer will be provided with system |
| Catalog | Manufacturer catalogue submitted in English |
| Remarks (any) | : N/A |
| Price | : Submitted. |
