Fluorescence Spectrophotometer |
FeaturesEnhanced sensitivity and dynamic range should enable fluorescence as well as bioluminescence, chemiluminescence, and electro-luminescence measurements.High-speed 3D scanning should enable rapid acquisition of 3D spectra.Spectral-Corrected Excitation and Emission spectra must be scanned.Fluorescence quantum yield and Fluorescence quantum efficiency measurements must be available. |
Technical Specifications: |
Brand | : Shimadzu Corporation, Japan |
Model | : RF-6000 |
High sensitivity, High Stability and High Speed: | Highest level SNR in its class; 1,000 or more (RMS) /350 or more (P-P) |
High speed scanning of 60,000 nm/min minimizes scan time or better. |
The long life of Xe lamp should be 2,000 hours. |
Extended range PMT should offer scan wavelength range to 900nm. |
Excellent Usability should have below features: | Easy-to-use RF software simplifies analysis. |
Validation routines included. |
The status bar in the software indicates lamp and accessory status. |
Large sample compartment for all analytical needs. |
Light Source should be 150W Xenon lamp or better |
Light Source Lifetime should be 2,000 hours or better |
Monochromator should be Blazed holographic grating, 1300 grooves/mm or better |
Wavelength Scale should be 200 to 900nm and 0 order or better |
Scanning Wavelength Range should be 200 to 900nm and 0 order or better |
Spectral Bandwidth: Excitation: 1.5, 3, 5, 10, 15, 20nm & Emission: 1, 3, 5, 10, 15, 20nm or better |
Resolution should be 1.0nm or less (Emission) or better |
Wavelength Accuracy should be within ±1.0nm or better |
Light Source Compensation: Monochromatic light monitoring ratio calculation |
Sensitivity: The SN ratio of the Raman line of distilled water should be 350 and more (P-P); 1,000 and more (RMS), Excitation 350nm, Spectral bandwidth 5nm, Response 2sec. |
Wavelength Scanning should be Max. 60,000nm/min or better |
Wavelength Slewing speed should be 60,000nm/min or better |
Sensitivity Selection should be 3 steps of High, Low and Auto or better |
Software Specifications: |
Conventional analysis: | Spectrum Mode should available Excitation spectrum, fluorescence spectrum, synchronous spectrum |
3D spectrum: | Repeated measurement in a specified time interval (3D excitation spectrum, 3D -fluorescence spectrum, 3D synchronous spectrum)Fluorescence spectrum measurement at a specified excitation wavelength interval (3D spectrum)Excitation/ fluorescence spectrum extraction |
Quantitation: | Quantitation using the peak / maximum value / area etc. of single wavelengths, multiple wavelengths (including single, double, and triple wavelength methods), and specified wavelength rangesK-factor method, single-point calibration curve method, and multi-point calibration curve method (1st, 2nd, and 3rd order function fitting, zero intercept can be specified)Photometric processing with user-defined functions (functions that use addition, subtraction, multiplication, and division can be embedded together with factors) |
Photometric: | Capturing the fluorescence intensity of single wavelengths and multiple wavelengths as well as peak /maximum value / area in specified wavelength rangesPhotometric processing with user-defined functions (functions that use addition, subtraction, multiplication, and division can be embedded together with factors) |
Time course: | Time course recording using single wavelength.Activity value calculationEvent recording of reagent additions during measurement |
Data processing: | Processing, data printing, point pick, peak pick, area calculation, constant calculation, data set calculation,1st to 4th order differentiation, smoothing, common logarithm transformation, natural logarithm transformation, reciprocal transformation, exponentiation, square root, index transformation of waveform data (spectrum/time course) |
Printing functions: | Report template creationPrinting using report templatesFile functions:Automatic conversion to CSV le and text le (.txt) formats (Only manual text conversion in the quantitation and photometric applications. |
Special analysis: | Fluorescence quantum yield:Fluorescence quantum yield calculation of unknown samplesDisplay and printing of results list of multiple samplesText conversion of spectrum data |
Accessories | a) Quartz Cell Polished- 2 Nos |
b) Non-Fluorescent Cell -2 Nos |
c) Solid (Powder) Sample Holder- 1 No.In addition to solid and powder samples, it must hold a liquid solution cell to measure the reflected fluorescent light. The angle of the holder should be designed to limit specular reflected excitation light from directly entering the fluorescence monochromator. Cutoff filters must be included to block excitation light and scattered light (includes U-340, IHU-310, L-42, Y-50, O-56, and R-60). d) An installation Table with cabinet and rack. |
Computer | Brand Computer Core i5, RAM 8 GB, SSD 500GB, DVD ROM drive, 18.5 inches LCD monitor, Original Windows with kit, mouse, and keyboard- 1Set. |
Online UPS | Online UPS: 3 KVA On-line UPS with 30 minutes backup-01 No. |
Printer | Printer: Laser Printer- 1No. |
Country of Origin | Japan. |
Make and assembly | Japan. |
Certification- Country of Origin | Yes |
Manufacturer Authorization Letter | Yes (On Manufacturer’s letterhead pad) |
Manufacturer Warranty and guaranty | Yes (On Manufacturer’s letterhead pad) |
Standard Warranty and guaranty | One Year |
Extended Service Warranty | Five years (including warranty period) |
Training | 3-days training on maintenance, trouble shooting, operation, and application of equipment must be provided by us after installation and commissioning and 2-days after 3-months of 1st schedule training at user site. We will train up the appointed scientists/experts/technician until ready for operation. |
Packing list | Packing list from manufacturer will be provided with system |
Catalog | Manufacturer catalogue submitted in English |
Remarks (any) | N/A |
Price | Included. |