| High-Performance Liquid Chromatography with PDA and Fluorescence Detector |
| FeaturesWith PDA & Fluorescence Detector must be capable to measure both qualitative and quantitative analysis.The system should be integrated with the Quaternary Gradient High-Performance Liquid Chromatograph.Must be capable of running the system with 66 MPa to perform Ultra High-Performance Liquid Chromatograph analysis method. |
| Technical Specifications: |
| Brand | : Shimadzu Corporation, Japan. |
| Model | : Advance i-Series. (LC-2050C 3D) |
| Injection Cycle Time: | Must be capable to 41 injections in 10 minutes or less. |
| Multi-Sample Processing with Ultrafast Injection Mechanism: | Increase in sample number demands shortening the analysis time and interval time (injection operating time). Faster injection cycle time of 14 seconds or less, which is almost thrice as fast as other systems. |
| Ultra-Low Carryover Performance Enables High-Sensitivity Analysis | carryover effects from residual samples reduced to nearly zero. Carryover specification was largely improved from 0.01% to 0.0025% and it provides highly precise quantitative performance, when analyzing complex samples. |
| Linearity and Reproducibility of Injection Volume | The injection volume linearity and reproducibility of the auto sampler must be improves the reliability of data for a wide range of injection volumes, from micro volumes of 1μL or less injected in the case of precious materials, such as biological samples, to 100μL volumes used for dissolution testing of pharmaceuticals or environmental waters. |
| Inter-System Reproducibility Enhances the Lab-to-Lab Data Compatibility: | The system must be capable system-to-system as well as single system reproducibility to assure data reliability. |
| Method Transfer between LCs regardless of Brands: | System must be capable to transferring methods from same brand LC and any branded LC system without changes settings. This helps ensure a smooth launch process after a new installation. |
| PDA detector for Target Components Confirmation Using Absorption Spectrometry: | Systems with a PDA detector ideal for quantitative testing, such as for non-pharmaceuticals and confirmation testing using absorption spectrometry. In addition to chromatograms from quantitative testing, the three-dimensional data obtained from the PDA detector, in terms of time, absorbance, and wavelength axes, also enables obtaining absorption spectra for each component from a single analysis and it allows you to compare purity indices to a standard spectrum. |
| Sensitivity Fluorescence Detector for Analysis of Anionic Surfactants: | Combining the system with a highly sensitive fluorescence detector ensures reliable detection and identification of trace components. |
| Solvent Delivery System (Quaternary System): | It must be a high-speed double plunger in parallel connection.Maximum operating pressure: 70 MPa or betterDegassing unit: Five Lines Mobile phase 4 + Rinse solution 1 (Volume 400 µL)Pumping method: Parallel-type double plungerPulsation: ≤ 0.1 MPa (1.0 mL/min,10 MPa, Water)Flow rate setting range: ≤ ±1% or ≤ ±2 µL/min, below whichever is greater (0.01 to 2 mL/min,1 to 40 MPa, specified condition), ≤ ±2% or ≤ ±2 µL/min, below whichever is greater (0.01 to 3 mL/min, 40 to 60 MPa, Specified condition)Flow rate precision: < 0.06 %RSD or < 0.02 minSD, below whichever is greater.Configuration: Four-solvent low-pressure gradientGradient / range of set concentrations: 0 to 100%, in 0.1% stepsGradient / concentration accuracy: ±0.5% (0.1 to 2 mL/min, 1 to 20 MPa, Specified condition)Gradient / concentration precision: ±0.1% (1 mL/min,10 MPa, Specified condition)Maximum pressure: 66 MPa (0.0001 to 3 mL/min)System Delay Volume: 460 µL, 650 µL and 1100 µL |
| Auto Sampler & Injector: | Injection method: Total-volume sample injectionInjection volume accuracy: ±1% (50 µL, N = 10)Injection volume setting range: 0.1 to 50 µL (Option: 0.1 to 100 µL, 1 to 500 µL, 1 to 2,000 µL)Injection volume reproducibility: RSD < 0.20% (5.0−2000 µL), RSD < 0.25% (2.0−4.9 µL), RSD < 0.5% (1.0−1.9 µL), RSD < 1.0% (0.5−0.9 µL). Cross-contamination: 0.0025% (Caffeine, Specified condition)Injection cycle time: Min. 14 sec (Specified condition)Samples for processing: 336 (1 mL), 216 (1.5 mL), 112 (4 mL), 4 (MTP/DWP)Sample Cooler: 4 to 45°C (Room temperature must be 15° to 30°C) Injection linearity: > 0.9999 (1 to 50 µL, Standard Sample Loop, specified condition), > 0.9999 (1 to 100 µL, 100 µL Sample Loop (Option), Specified condition) |
| Column Oven: | Heating and cooling method: Forced air circulation method.Containable column size: 6 pieces at 10 cm max., 3 pieces at 10 cm to 30 cm Temperature control range: Room temperature- 12 to 90°C, Setting range 4 to 90°C.Temperature control precision: ±0.1°C or less. Temperature stability: ±0.8°C Flow rate switching valve: One pc |
| PDA Detector: | Wavelength range: 190 to 800 nmSpectral resolution: 1.4 nmSlit width: 1.2 nm, 8 nm.Device resolution: 0.6 nm/pixel or betterNumber of photodiode array elements: 1024 or moreWavelength accuracy: ≤ ±1 nmNoise level: ≤ ±3 × 10−6 AU (250 nm, reference: 350 nm. Drift: ≤ 500 × 10−6 AU/h (250 nm, reference: 350 nm. Linearity: Up to 2 AU (5%). Sampling rate: Up to 100 HzLight source: Deuterium (D2) lamp & Tungsten (W) lampFlow cell: 10μL (10mm, TC) 12 MPaOption cell: High-Speed: 8μL (10 mm, TC) & Semi-micro: 2.5μL (5 mm, TC) |
| Fluorescence Detector: | Must be possible to monitor dual wavelength simultaneously. Light Source: Xenon lamp, low-pressure mercury lamp (To check wavelength accuracy)Wavelength Range: 0.200 to 750 nmSpectral Bandwidth: 20 nmWavelength Accuracy: ±2 nm or betterWavelength Precision: ±0.2 nm or betterCell Capacity: 12μL, 2 MPa (approx. 20kgf/cm2), SUS316L, PTFE (Fluororesin), quartz. Cell Temperature Control Range: 4°C to 40°C, 1°C stepsCell Temperature Setting Range: Below ambient -10°C to 40°CFunctions: Four-wavelength detection, wavelength scanningSafety Measures: Liquid-leakage sensorOperating Temperature Range: 4 to 35°C |
| Compatible Software Package (Package must include the followings): | Data acquisition for signals and spectraPeak integration and qualificationControl of components like auto sampler, pump, detector, degasserStorage and management of chromatographic dataTotal Compatible with Regulatory Compliance like FDA 21 CFR 11 compliance (including electronic record and electronic signature)Indication for exceeding pressure |
| Online Degasser: | On-line Channel Degasser: 5 or more Degassed flow-line capacity: 400µl or higher |
| The integrated type of Quaternary Gradient HPLC Should be configuring as follows | Solvent Delivery System- 1No. PDA Detectors- 1No. Fluorescence Detectors- 1No. Auto Injector- 1No. Analytical Columns different size columns for relevant research- 04Nos. Column Oven- 1No. Necessary Accessories- 1Set. Compatible Software- 1No. Online Degasser- 1No. Reservoir Tray- 1No. Reservoir Bottle 1000 mL- 2Nos. Reservoir Bottle 500 mL- 2Nos. Solvent Filtration Unit with Pump- 1Set. Membrane filter 0.22 µm Pack of 200pcs- 3Pack. Membrane filter 0.45 µm Pack of 200pcs- 3Pack. |
| Accessories: |
| Chemical & Reagent for HPLC: | HPLC Grade Methanol Pack of 2.5L- 2Pack. HPLC Grade Acetonitrile Pack of 2.5L- 2Pack. Isopropyl alcohol Pack of 2.5L- 1Pack. Acetic Acid Pack of 1L- 1Pack. Formic Acid Pack of 0.5L- 2Pack. Ammonium Format Pack of 500gm- 1Pack.An installation Table with cabinet and rack. |
| Computer | Brand computer, Intel Core i7, 8GB RAM, 1TB SSD Hard disk, DVD-RW ± Dual, Keyboard, Mouse & Pre-installed Microsoft Original Windows-10 Professional, 24” full screen monitor- 1No. |
| Online UPS | Reputed brand On-Line UPS 6KVA with 60 minutes back-up- 1no. |
| Printer | Laser Jet Printer with auto duplex system- 1No. |
| Country of Origin | Japan. |
| Make and assembly | Japan. |
| Certification- Country of Origin | Yes |
| Manufacturer Authorization Letter | Yes (On Manufacturer’s letterhead pad) |
| Manufacturer Warranty and guaranty | Yes (On Manufacturer’s letterhead pad) |
| Standard Warranty and guaranty | One Year |
| Extended Service Warranty | Five years (including warranty period) |
| Training | 5-days training on maintenance, trouble shooting, operation, and application of equipment will be provided by manufacturer traind engineer after installation and commissioning and 3-days after 3-months of 1st schedule training at user site. We will train up the appointed scientists/experts/technician until ready for operation. |
| Packing list | Packing list from manufacturer will be provided with system |
| Catalog | Manufacturer catalogue submitted in English |
| Remarks (any) | N/A. |
| Price | Included. |
